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immunomagnetic separation in tanzania

Unexpected results from large-scale cryptosporidiosis ...

A modified immunomagnetic separation (IMS) procedure was used (GC-Combo; Dynal Invitrogen, Oslo, Norway) as previously described (Robertson et al., 2006), in which a 1 ml suspension of fecal material was mixed (end-over-end rotation) for 1 hour with 100 μl of each buffer provided in the kit and 15 μl beads coated with anti-Cryptosporidium oocyst antibody. DNA was then extracted from the ...

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Immunomagnetic Separation - an overview ScienceDirect Topics

Immunomagnetic separation (IMS) for isolation of Listeria serves as the basis for assays that are ideally suited to food industry applications. These include hazard analysis and critical control point (HACCP) programmes as well as in-process and finished product testing. IMS can be integrated into such testing in

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Immunomagnetic Separation - an overview ScienceDirect Topics

Immunomagnetic separation (IMS) for isolation of Listeria serves as the basis for assays that are ideally suited to food industry applications. These include hazard analysis and critical control point (HACCP) programmes as well as in-process and finished product testing. IMS can be integrated into such testing in

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Bioline International Official Site (site up-dated regularly)

Tanzania Journal of Health Research, Vol. 10, No. 3, July, 2008, pp ... of the samples on CT-SMAC-BCIG agar were inoculated onto mTSB and about 5ml of each broth culture was used for immunomagnetic separation (IMS). IMS with magnetic beads coated with antibody to O157 (Dynal) was performed according to the instructions of the manufacturer. The concentrates were inoculated

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Isolation of B-cells using Miltenyi MACS bead isolation kits

Immunomagnetic Separation / methods* Grant support This study was supported by Stellenbosch University; Stellenbosch University Immunology Research Group and the Molecular Biology and Human Genetics Clinical Group; National Research Foundation; South African Medical Research Council. AGL is supported by the NRF-CSUR (Grant Number CSUR60502163639) and the Centre for Tuberculosis

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Retrieval of full-length functional genes using ...

Meiring T(1), Mulako I, Tuffin MI, Meyer Q, Cowan DA. Author information: (1)Institute for Microbial Biotechnology and Metagenomics, University of the Western Cape, Cape Town, South Africa. Numerous gene-specific PCR methods have been developed for the cultivation-independent discovery of novel ...

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An immunomagnetic separation-real-time PCR system for the ...

2014-04-03  Alicyclobacillus acidoterrestris is the most important spoilage species within the Alicyclobacillus genus and has become a major issue in the pasteurized fruit juice industry. The aim of this study was to develop a method combining immunomagnetic separation (IMS) with real-time PCR system (IMS-PCR) for rapid and specific detection of A. acidoterrestris in fruit products.

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(PDF) Immunomagnetic separation of Escherichia coli O157 ...

Immunomagnetic separation (IMS) has been extensively used for the isolation of E. coli O157:H7 from food and stool samples but not for samples such as wastewater. In this study the IMS method was ...

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Immunomagnetic separation of Escherichia coli O26, O111 ...

The aim of this study was to isolate Escherichia coli O26, O103, O111 and O145 from 745 samples of bovine faeces using (i) immunomagnetic separation (IMS) beads coated with antibodies to ...

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Sextuplex PCR combined with immunomagnetic separation and ...

2017-03-01  Immunomagnetic separation (IMS) uses magnetic nano-beads coated with specific antibodies to capture target pathogens. IMS has been proven to be an effective technique for the selective isolation and concentration of target bacteria from the sample matrix, thereby reducing analysis time and improving detection limit.

More

Micro- and nanotechnology-based approaches to detect ...

2017-01-01  Immunomagnetic separation and detection scheme. Immunomagnetic beads are mixed with a mixed sample containing target (ovals) and nontarget (circles) organisms (Step 1). After mixing, magnetic separation and washing (Step 2) only target organisms are bound to the beads. This can be followed by labeling with secondary antibodies/labels (Step 3).

More

Unexpected results from large-scale cryptosporidiosis ...

A modified immunomagnetic separation (IMS) procedure was used (GC-Combo; Dynal Invitrogen, Oslo, Norway) as previously described (Robertson et al., 2006), in which a 1 ml suspension of fecal material was mixed (end-over-end rotation) for 1 hour with 100 μl of each buffer provided in the kit and 15 μl beads coated with anti-Cryptosporidiumoocyst antibody.

More

Retrieval of full-length functional genes using ...

Meiring T(1), Mulako I, Tuffin MI, Meyer Q, Cowan DA. Author information: (1)Institute for Microbial Biotechnology and Metagenomics, University of the Western Cape, Cape Town, South Africa. Numerous gene-specific PCR methods have been developed for the cultivation-independent discovery of novel ...

More

Methods for the detection of Cryptosporidium and Giardia ...

2018-08-01  Immunomagnetic separation and immunofluorescence assays have been criticized for being time-consuming, labour-intensive, expensive and for requiring a high level of analytical expertise (LeChevallier et al., 2003). However, the application is much better than traditional methods and their application is far more precise. 2.2.2.

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Isolation and Culture of Fibroblasts, Vascular Smooth ...

A, Overview of FCM histograms before (upper row) and after (lower row) immunomagnetic cell separation for fibroblasts (anti-CD 90, first column), ECs (anti-vWF, second column), and VSMCs ...

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Isolation of a Pandemic O3:K6 Clone of a Vibrio ...

Recovery of V. parahaemolyticusVP47 inoculated into APW containing seafood by the K6-specific immunomagnetic separation method with or without enrichment culture of the immunomagnetic particles (IMP) Tomoyasu's procedure included overnight incubation of the washed IMP in an enrichment broth before inoculation onto TCBS agar.

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An immunomagnetic separation-real-time PCR system for the ...

2014-04-03  Alicyclobacillus acidoterrestris is the most important spoilage species within the Alicyclobacillus genus and has become a major issue in the pasteurized fruit juice industry. The aim of this study was to develop a method combining immunomagnetic separation (IMS) with real-time PCR system (IMS-PCR) for rapid and specific detection of A. acidoterrestris in fruit products.

More

Isolation of a Pandemic O3:K6 Clone of a Vibrio ...

Recovery of V. parahaemolyticusVP47 inoculated into APW containing seafood by the K6-specific immunomagnetic separation method with or without enrichment culture of the immunomagnetic particles (IMP) Tomoyasu's procedure included overnight incubation of the washed IMP in an enrichment broth before inoculation onto TCBS agar.

More

Bioline International Official Site (site up-dated regularly)

Tanzania Journal of Health Research, Vol. 10, No. 3, July, 2008, pp ... of the samples on CT-SMAC-BCIG agar were inoculated onto mTSB and about 5ml of each broth culture was used for immunomagnetic separation (IMS). IMS with magnetic beads coated with antibody to O157 (Dynal) was performed according to the instructions of the manufacturer. The concentrates were inoculated

More

Retrieval of full-length functional genes using ...

Meiring T(1), Mulako I, Tuffin MI, Meyer Q, Cowan DA. Author information: (1)Institute for Microbial Biotechnology and Metagenomics, University of the Western Cape, Cape Town, South Africa. Numerous gene-specific PCR methods have been developed for the cultivation-independent discovery of novel ...

More

Sextuplex PCR combined with immunomagnetic separation and ...

2017-03-01  Immunomagnetic separation (IMS) uses magnetic nano-beads coated with specific antibodies to capture target pathogens. IMS has been proven to be an effective technique for the selective isolation and concentration of target bacteria from the sample matrix, thereby reducing analysis time and improving detection limit.

More

Combined use of an immunomagnetic separation method and ...

Subsequently, a combination of immunomagnetic separation, cefixime–tellurite–sorbitol MacConkey (CT‐SMAC) plating and immunoblotting with specific O157 antibodies allowed the detection, enumeration and isolation of E. coli O157 strains in human, swine and cattle wastewaters, which presented values of 02, 04, and 10 log 10 ml −1 units, respectively. Some of the isolates carried ...

More

Micro- and nanotechnology-based approaches to detect ...

2017-01-01  Immunomagnetic separation and detection scheme. Immunomagnetic beads are mixed with a mixed sample containing target (ovals) and nontarget (circles) organisms (Step 1). After mixing, magnetic separation and washing (Step 2) only target organisms are bound to the beads. This can be followed by labeling with secondary antibodies/labels (Step 3).

More

Rapid detection of food-borne Salmonella contamination ...

2018-04-01  Immunomagnetic separation (IMS) of Salmonella in food samples Fresh pork and whole milk was purchased from a local grocery store and used for rapid detection of Salmonella. Salmonella cultures were decimally diluted in PBS for the further addition of milk pork and beef samples.

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Methods for the detection of Cryptosporidium and Giardia ...

2018-08-01  Immunomagnetic separation is a purification procedure that in the current application with Cryptosporidium and/or Giardia uses microscopic, magnetically responsive particles coated with an antibody targeted to react with these specific pathogens.

More

Isolation and Culture of Fibroblasts, Vascular Smooth ...

A, Overview of FCM histograms before (upper row) and after (lower row) immunomagnetic cell separation for fibroblasts (anti-CD 90, first column), ECs (anti-vWF, second column), and VSMCs ...

More

Combined use of an immunomagnetic separation method and ...

Subsequently, a combination of immunomagnetic separation, cefixime–tellurite–sorbitol MacConkey (CT‐SMAC) plating and immunoblotting with specific O157 antibodies allowed the detection, enumeration and isolation of E. coli O157 strains in human, swine and cattle wastewaters, which presented values of 02, 04, and 10 log 10 ml −1 units, respectively. Some of the isolates carried ...

More

Ultrasensitive Ebola Virus Detection Based on ...

The 2014–16 Ebola virus (EBOV) outbreak in West Africa has attracted widespread concern. Rapid and sensitive detection methods are urgently needed for diagnosis and treatment of the disease. Here, we propose a novel method for EBOV detection based on efficient amplification of electroluminescent nanospheres (ENs) coupled with immunomagnetic separation. Uniform ENs are made by

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Development of peR-based detection assays for Legionella ...

Alternative methods of target isolation, e.g. immunomagnetic separation (IMS) and solid-phase sequence capture have proven useful for detection of various pathogenic bacteria and viruses from PCR inhibitory substances (Hultman et aI, 1989; Rimstad et aI, 1990; Luk et aI, 1991; Cudjoe et aI, 1994; Olsvik et aI, 1994; Mangiapan at aI, 1996; Pyle et aI, 1999). The former is based on isolation of ...

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1, 1 3, - MDPI

Another technique to shorten the isolation stage is immunomagnetic separation (IMS), in which the selective enrichment stage can be replaced with nongrowth-related procedures [24]. In this approach, superparamagnetic particles (such as iron oxide-coated polystyrene beads) coated with target organism-specific antibodies are used to isolate the pathogens from the aqueous solution containing a ...

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Immunomagnetic nanoparticle based quantitative PCR for ...

Made available by U.S. Department of Energy Office of Scientific and Technical Information ...

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Detection of Escherichia coli serogroups O26, O103, O111 ...

Aims: The aim of this study was to isolate Escherichia coli O26, O103, O111 and O145 from 745 samples of bovine faeces using (i) immunomagnetic separation (IMS) beads coated with antibodies to lipopolysaccharide, and slide agglutination (SA) tests and (ii) PCR and DNA probes for the detection of the Verocytotoxin (VT) genes. Methods and Results: IMS‐SA tests detected 132 isolates of ...

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Methods for the detection of Cryptosporidium and Giardia ...

2018-08-01  Immunomagnetic separation is a purification procedure that in the current application with Cryptosporidium and/or Giardia uses microscopic, magnetically responsive particles coated with an antibody targeted to react with these specific pathogens.

More

MDPI Article Reprints Order

Article: Detection of E. coli O157:H7 in Food Using Automated Immunomagnetic Separation Combined with Real-Time PCR Authors: by Ji Young Park , Min-Cheol Lim , Kisang Park , Gyeongsik Ok , Hyun-Joo Chang , Nari Lee , Tae Jung Park and Sung-Wook Choi

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209/MDCT ATCC ® CRL-3250™ Mus musculus kidney

Pizzonia JH, et al. Immunomagnetic separation, primary culture, and characterization of cortical thick ascending limb plus distal convoluted tubule cells from mouse kidney. In Vitro Cell Dev. Biol. 27A(5): 409-416, 1991. PubMed: 1649164. Permits: These permits may be required for shipping this product: Customers located in the state of Hawaii will need to contact the Hawaii Department of ...

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Matthew Leslie - Technologist - Sanofi Pasteur LinkedIn

• Proficient at PCR, ELISA, Immunoblotting and Immunomagnetic Separation Assays • Organized weekly findings in detailed written reports and Excel spreadsheets and communicates results directly to supervisors • Participating in a Collective Research Agreement with Dupont Canada, involving the validation of a real-time PCR equipment specific for the detection of VTEC Show more Show less ...

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